Efficacy of the in vitro splitting of human preimplantation embryos from ART programs

Background/aim: The aim of this study was to evaluate the efficiency in vitro embryo splitting (IES) procedures. We also assessed quality blastocysts developed from embryos obtained different sources. Materials and methods: Good at 6-8-cell stages were categorized according their fertilization sources: 1) frozenwarmed donated embryos, 2) chromosomally abnormal 3) parthenogenetic 4) derived matured oocytes (rescue IVM). After IES, developmental assessed. Furthermore, evaluated by Hoechst propidium iodide (PI) staining. Results: data showed a high rate both frozen-warmed after IES (140% 71.7%, respectively), followed (96.8% 52.5%, respectively). Results PI staining that mean ± SD cell numbers control group higher (113.11 16.01) than twins A (donor blastomeres 58 12.2) B (recipient 50.4 8.5), respectively. Conclusion: Chromosomally normal enrolled are more potent develop into viable blastocysts. For research purposes, 1PN 3PN best options for procedures, regardless poor